Increasingly, natural products have potential for the treatment of cancer. The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of some medicinal plants against escherichia coli, salmonella typhimurium, staphylococcus aureus and enterococcus sp. Antimicrobial activity, toxicity and phytochemical. Mic value shows that these plant extracts have the least antimicrobial activity. Feb 09, 2015 the observed mic and mbc values of the plant extracts in this study are in a range or lower than the values of other plant extracts reported from different countries 15, but are higher than those values that have been reported in a number of reports 11, 16, 17. The effect of various plant extracts was measured on several important oxidation indicators. Satish 1department of studies in applied botany and biotechnology, university of mysore, manasagangotri, mysore570 006 2herbal drug technology laboratory, department of studies in microbiology. Sage and yarrow extracts did not present any antimicrobial activity. Evaluation of antibacterial activity of some medicinal plants. Assessment of antioxidant activity of plant extracts by.
The results showed that extracts of mint, lemon balm and coriander among others inhibited the growth of e. Aug 20, 20 in addition to antioxidant activity, plant phenolic compounds, such as flavonoids and tannins, have been shown to possess antimicrobial activities 1618. Antibacterial and antifungal activities of thespesia. Antibacterial activity of aquilaria crassna leaf extract. Studies on the antimicrobial activity of essential oils from t. Antimicrobial activity of single and combined extracts of. Antimicrobial activity of selected medicinal plants. Antimicrobial activity of some plant extracts against. Jun 03, 2015 evaluation of antibacterial activity of some medicinal plants used in sudanese folk medicine for treatment of gastrointestinal tract infections. Among the four extracts of neem, petroleum ether and chloroform extract showed strong antimicrobial activity against s. The use of plant extracts with known antimicrobial properties, can be of great significance in therapeutic treatments.
Boiled water extract of neem leaf showed moderate zone of inhibition against escherichia coli 10 mm and. Antimicrobial activity of some indian medicinal plants. The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of ocimum basilicum l. The antimicrobial potential of the above plant extracts was seen against the test organism using agar gel diffusion susceptibility test by standard technique of opara and anasa19939. Antibacterial activities of the extracts were first screened by agarwell diffusion method as described previously. The three bacteria used in the current study were all gramnegative.
Antibacterial activities of some borneo plant extracts. Antimicrobial activity of some important medicinal plant against plant and human pathogens 12b. The differences of solvent related activity could be speculatively explained by the insolubility of active compounds in water. Microorganisms dichloromethane extracts methanol extracts ac mp sc ta ro pg ac mp sc ta ro pg.
The present study aimed at evaluating the in vitro antimicrobial activity of plant allium sativum, zingiber officinale, caryophyllus aromaticus, cymbopogon citratus, mikania glomerata and psidium guajava extracts against grampositive and gramnegative bacterial strains isolated from human infections. The most active plant was torilis anthriscus especially in relation to pseudomonas glycinea. Punica granatum, citrullus colocynthes, curcuma long, albizia lebbeckandazardirachta indicaare used in traditional medical practice in sudan for the treatment of gastrointestinal tract infections. Antibacterial activity the agar well diffusion method was performed to exploit antibacterial potential of used extracts 8. An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. The mic testing was performed for all the plant extracts that were judged as active inhibition zone 7 mm against at least one test organism by agarwell diffusion method. Issn phytochemical and antimicrobial evaluation of plant. Bioactive aqueous extracts of twelve yucatecan plants. The main objectives of this study were to examine antibacterial activity of the aquilaria crassna leaf extract against staphylococcus epidermidis and its underlying mechanism. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Water extracts of green tea leaf at boiling and room temperature showed 17. Antimicrobial activity of medicinal plants against human. Antimicrobial activity of ethanol leaf extracts of. Satish 1department of studies in applied botany and biotechnology, university of mysore, manasagangotri, mysore570 006 2herbal drug technology laboratory, department of.
Upon heating, it was rinsed for 24 h at room temperature. Screening of antibacterial activity of the medicinal plant. Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1. Antimicrobial activity of some important medicinal plant. Pdf selected cameroonian plants have been investigated for their antifungal and antibacterial activity against five species. We aimed to find the in vitro antibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of nepal against clinical and 2 reference bacterial. Antibacterial activity of medicinal plant extracts article pdf available in brazilian journal of microbiology 384. Antiplasmodial, antimicrobial and cytotoxic activities of. In vitro antimicrobial activity of some medicinal plants. Feb 27, 2016 antimicrobial activity of various plants extracts 1. Antimicrobial activity, toxicity and phytochemical screening. Antimicrobial activity of medicinal plant extracts n g r am et iv b c ada zwetlana, nandini m, kusuma dorcas abstract various types of antibacterial substances are present in higher plants. Plant extracts with biofilm inhibitory activity biofilms are the default mode of life for many bacterial species and biofilmbased infections cause harm to millions of humans annually. Aloe vera, antimicrobial activity, sap, leaves, minimum inhibitory concentration introduction.
Antimicrobial activity of some plant extracts against bacterial strains. Biochemistry graduates to have results from their final year. Four different solvents were used for the extraction. The antimicrobial compounds produced by plants are active against plant and human pathogenic microorganisms. In relation to tetracycline activity, ethanol and ethyl acetate extracts showed similar or same activities while the water activity was higher than standard 100. Antimicrobial activity of selected medicinal plants against.
Plant extracts were added based on plackettburman design by varying the composition of chosen independent variables. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al. Biological screening of various medicinal plant extracts. These extracts were tested in vitro against 10 bacteria and 4 yeasts strains by the disc diffusion method. New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5. Evaluation of antibacterial activity of some traditionally. Antibacterial activities of some plant extracts utilized. Antimicrobial activity of crude extracts from plant parts and corresponding calli of f bixa orellana l. Pdf antimicrobial activities of various medicinal and commercial. All in all, data about specific antibiotic properties of coumarins are. Studies on in vitro antimicrobial activity of ethanol extracts of rauvolfia tetraphylla. All the plant extracts were screened for their antimicrobial activity. Pdf antimicrobial activity of medicinal plant leaf.
Antioxidant, antihypertensive, antihyperglycemic, and antimicrobial. The highest antibacterial activity of crude eos was observed in p. Boiled water extracts of guava leaf showed the largest zone of inhibition 22 mm against v. None of the aqueous extracts showed antimicrobial activity. In vitro antifungal activity of dichloromethane and methanol extracts mgml. Different parts of the plants were collected, air dried, and powdered in a homogenizer, and 10 g of each plant was used for aqueous and ethanol extraction. Antimicrobial activity of plant extract was considered to be good if its mic was less than 100. Evaluation of antibacterial activity of some medicinal. Association of antibiotics and plant extracts showed synergistic antibacterial. It was found that most plant extracts studied had antibacterial and antifungal activities. Antimicrobial potential of some plant extracts against. The extract of three medicinal plants used in nigerian folk medicine showed a highly antibacterial activity against some food borne pathogens. Antibacterial activity of extracts prepared from tropical and subtropical plants on methicillinresistant staphylococcus aureus.
Antibacterial and antifungal activities of thespesia populnea. Chemical composition and antibacterial activity of essential. Abts, dpph and frap assays are widely used to determine the antioxidant capacity in plant extracts because of their simplicity, stability and accuracy. Antimicrobial activity of wild olive crude extracts in vitro. Introduction even though pharmacological industries have produced a number of new antibiotics in the last three decades, resistance to these drugs by microorganisms has increased. Antibacterial activities of plant extracts were compared with those of standard antibiotics. In this study, nine plants extracts were screened for potential antibacterial activity table. Plant extracts with biofilm inhibitory activity biofilms are the default modeoflife for many bacterial species and biofilmbased infections cause harm to millions of humans annually. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Antimicrobial assay the antimicrobial activity of the plant extracts was evaluated using agar well diffusion method 21 with minor modifications.
The methanolic extract phyllanthus niruri stone breaker showed the maximum activity against staphylococcus sp. Species aegopodium podagraria, daucus carota, heracleum sphondylium and pimpinella. The methanol and aqueous extracts from different plant parts peel of fruits, rhizome, fruits, leaves, and gum respectively at a concentration of 100 mgml, were evaluated against 20 clinical. Phytochemical analysis of phyllanthus amarus extracts s. Results of antimicrobial activity of the five plant extracts can suggested that s. Antimicrobial activity of five herbal extracts against multi drug. Antimicrobial activity of crude extracts of three medicinal. To determine antibacterial activity of water, oil and methanol extracts of guava psidium guajava, green tea camellia sinensis, neem azadirachta indica and marigold calendula officinalis against different species of bacteria, pseudomonas spp. About 250,000 living plant species contain a much greater diversity of bioactive compounds than any chemical library made by humans but only few plant species have been systematically investigated for the presence of bioactive compounds wakdikar, 2004. Antiplasmodial, antimicrobial and cytotoxic activities of extracts from selected medicinal plants growing in tanzania.
Studies on some plant extracts for their antimicrobial. All plant extracts methanol and aqueous show significant antimicrobial activity against both test bacteria s. Antibacterial activities of some plant extracts utilized in popular medicine in palestine 102 1. Antimicrobial activity of medicinal plants against human pathogenic bacteria 657 inflammatory properties of plants mahesh and satish, 2008. Antimicrobial activity of aqueous and organic extracts. The activity of crude essential oils against the three bacteria isolates was within the extremely sensitive category diameters of the inhibition zone larger than 20 mm after 24 and 48 hours of incubation table 1. The results indicated that the methanol extracts of o. To prepare plant extracts three different solvents were used. Fresh leaves of eleven different plant species free from. Antibacterial activity of some plant extracts nihal dog ru oz 1, zuhal zeybek 1, ali karagoz 2 1 istanbul university, faculty of science, department of b iology, 344, vezneciler, istanbultu.
Antimicrobial activity of crude extracts of some plant leaves. In vitro investigation of antimicrobial activities of. Table 2 mic of plant extracts against the microorganisms by micro dilution broth assay. Studies to determine the antibacterial acitivities of. A study of antimicrobial activity of few medicinal herbs. Chemical composition and antibacterial activity of. The present investigation was focused on screening of ethanol leaf extracts of catharanthus roseus from saudi arabia for its antibacterial and antifungal activity against number of pathogenic microorganisms. Pdf antibacterial activity of medicinal plant extracts. Screening of in vitro antimicrobial activity of plants used in traditional. The high temperature during soxhlet extraction in our study might be. Antibacterial activity of plant extracts of alstonia scholaris.
Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Evaluation of antibacterial activity of these plant extracts was recorded in table 2 and illustrated in fig. Minimum inhibitory concentration mic determination. The plant extracts were obtained by using ethanol and the concentration of the extract was 500600 mg l1 and the the inhibition zone was measured after 24 h at 30c. General antimicrobial activity was documented in woodruff galium odoratum extracts 224.
Journal of biologically active products from nature. The methanolic extract of caryophyllus aromaticus presented the highest antis. All extracts were stored at 4c till further analysis. Plant derived medicines have made significant contributions towards human health and plants and are a great source of novel drug compounds. Antimicrobial activity of medicinal plant extracts and. The study of antioxidant and antimicrobial activity of extracts for meat marinades 9690 romanian biotechnological letters, vol. Pdf antimicrobial activity of single and combined extracts of. Antimicrobial activity of various plants extracts was compared with commercially available antibiotics. Antimicrobial activity of medicinal plant leaf extracts. The leaves were washed thor oughly 2 to 3 times with running tap water, leaf material.
Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. The methanolic extract of leaf of the plant azadiracta indica, acacia nilotica and witania somnifera showed significant. Ates and others published antimicrobial activities of various medicinal and commercial plant extract find, read and cite all the. Many methods for detecting such activity are available, but since they are not equally sensitive or even based on the same. Dried leaves of plants were mechanically ground, and 2 g of plant was extracted with 20 ml of acetone or chloroform or methanol, then it was gently heated. Biological screening of various medicinal plant extracts for. The study of antioxidant and antimicrobial activity of. The worldwide increase of multidrug resistance in both community and healthcare associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives.
Plant extracts since many of the essential oils inhibited the growth of e. Five plant species were investigated to evaluate their antibacterial activity against food poisoning bacteria including two strains of gram positive bacteria b. Research article open access antibacterial activity of. Aquilaria crassna pierre ex lecomte has been traditionally used in thailand for treatment of infectious diseases such as diarrhoea and skin diseases for a long time. Antibacterial activity of aqueous extract of calotropis. Antimicrobial activities of the crude ethanolic extracts of five plants were screened against multidrug resistant mdr strains of. Antimicrobial activity of various extracts of ocimum. Antimicrobial activity of plants can be dedicated by observing the growth response of various microorganisms to those plant tissues or extracts, which are placed in them. Wild olive crude extracts were screened for antibacterial activity against five different bacterial human pathogens.
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